Reduced titer of BNYVV in transgenic sugar beets expressing the BNYVV coat protein

Summary Rhizomania is a disease of sugar beet caused by the furovirus beet necrotic yellow vein virus (BNYVV). Coat protein mediated resistance has been reported for a number of viral diseases. This approach to virus resistance was therefore attempted for control of rhizomania. Two constructs of the coat protein gene of BNYVV were introduced into sugar beet by Agrobacterium-mediated transformation. The mRNA level was estimated to be 0.01% of the poly A⁺ RNA. Expression of the coat protein gene was under the detection limit of our western blotting protocol i.e. below 0.01 g/50 g (0.02% of the total soluble protein). One transformation event per construct was tested in a greenhouse assay and in rhizomania infested soil in a field trial. In the greenhouse assay, transgenic plants showed a strong reduction of virus multiplication when compared to non-transgenic plants. This result was confirmed in the field trial, where a significant difference in virus multiplication was shown between plants with and without the coat protein gene.     

塔额盆地引进甜菜品种比较试验初报

对引进33个甜菜品种进行比较试验,结果表明：kuhn1016、CN07、hx905、ST21916、kuhn1011、MA096、ST21015七个品种折合667m2产量5506kg以上,比对照增产4.36%以上;cofco8010、hx820、kuhn1001、kuhn1003、kuhn1010、ST21016六个品种含糖率在18.04%以上,比对照提高1.75度以上;cofco9016、cofco8010、hx905、kuhn1010、SD13806、ST21916、ST21015、ST21016八个品种产糖量较对照增产11.18%以上;综合各品种主要经济性状及抗病性,cofco8010、hx905、kuhn1010、ST21916、ST21015、ST21016六个品种较适合塔额盆地的生态环境,适宜在该地区推广应用。     

齐齐哈尔市甜菜小垄密植高产栽培技术

针对齐齐哈尔市甜菜生产现状,提出适合齐齐哈尔市甜菜生产的小垄密植综合高产栽培技术。     

我国甜菜栽培模式研究进展

综述了我国甜菜栽培模式的发展现状,从不同栽培方式、播期、合理密植、合理施肥、合理灌水等方面阐述了对甜菜产量、质量的影响。     

分子标记在甜菜离子注入诱变育种中的初步应用

利用经过离子注入诱变产生的早熟、高糖突变体为材料,结合RAPD标记分析进一步验证了离子注入诱变应用在甜菜上的有效性;通过分析材料间DNA水平上的差异,得到了甜菜品系7208、W11、S10之间有差异的引物;建立了7208、W11和S10的指纹图谱库,为新标记的创制奠定基础;为分子标记辅助选择育种提供物质保障和技术支撑;同时也为拓宽甜菜种质提供有效的方法。     

SODm叶面肥在甜菜上的应用效果

试验探讨了甜菜施用SODm叶面肥对其产量和含糖率的影响,试验结果表明：苗期喷施SODm叶面肥可以促进甜菜生长发育,提高甜菜含糖率,增产作用明显,经济效益显著,含糖率提高0.6度,块根增产13.6%。     

L'electrode potassium pour l'analyse des tissus vegetaux (The potassium electrode for plant tissue testing)

Abstract

A potassium specific‐ion electrode equiped with a “sensing module” was compared with atomic absorption for potassium determination in plant tissue. The potentiometric readings were stable and reproducible, and were of the same order of magnitude as those obtained by atomic absorption. The correletion coefficient obtained between the two analytical methods were highly significant; but it was higher for corn leaves, sugarbeet leaf‐blades and petioles respectively.     

甜菜施用生物钾肥试验简报

甜菜施用生物钾肥对比试验结果显示，根产量、含糖率、产糖量分别比对照提高１４％、０．５度和１５％，根腐病发病率比对照降低了１０．８％，是一项经济实用的施肥措施。